Evaluation of Drug Responses to Human β2AR Using Native Mass Spectrometry

We aimed to develop a platform to rapidly investigate the responses of agonists and antagonists to G-protein-coupled receptors (GPCRs) using native mass spectrometry (MS). We successfully observed the ligand-bound human β2 adrenergic receptor (hβ2AR); however, it was challenging to quantitatively discuss drug efficacy from MS data alone.

Development and validation of a rurality index for health care research in Japan: A modified Delphi study

Abstract Objectives Rural–urban healthcare disparities exist globally. Various countries have used a rurality index for evaluating the disparities. Although Japan has many remote islands and rural areas, no rurality index exists. This study aimed to develop and validate a Rurality Index for Japan (RIJ) for healthcare research.

COVID-19, young people, and suicidal behaviour

Sheri Madigan and colleagues'1 systematic review evaluates whether the COVID-19 pandemic has affected paediatric emergency department visits for attempted suicide, self-harm, and suicidal ideation. They report that the combined rate of emergency department visits for attempted suicide and suicidal ideation increased among girls (rate ratio 1·39, 90% CI 1·04–1·88), whereas there was no significant change among boys (1·06, 0·92–1·24).1 We were interested in this difference between the sexes because the COVID-19 pandemic appears to have had a greater impact on the behaviour of girls than boys in Japan. Therefore, we decided to analyse cases of death by suicide among adolescents and young adults in Japan.

Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis.

Heterosis contributes greatly to the worldwide agricultural yield. However, the molecular mechanism underlying heterosis remains unclear. This study took advantage of Arabidopsis intraspecific hybrids to identify heterosis-related metabolites. Forty-six intraspecific hybrids were used to examine parental effects on seed area and germination time.