長寿科学研究室

研究業績

2019年

  1. Yamakami Y, Morino K, Takauji Y, Kasukabe R, Miki K, Hossain MN, Ayusawa D and Fujii M. Extract of Emblica officinalis enhances the growth of human keratinocytes in culture. J Integrative Medicine. 2019 Jan.
  2. Arai R, En A, Takauji Y, Maki K, Miki K, Fujii M and Ayusawa D. Lamin B receptor (LBR) is involved in the induction of cellular senescence in human cells. Mech Ageing Dev. 2019 Jan.

2018年

  1. Awaya A. Development of Many So-Called Autoimmune Diseases Including Various Vasculitis Syndromes May be Commonly Triggered by Pollen Exposure. Jacobs Journal of Allergy & Immunology. 2018 Dec.
  2. Asano G, Kubota S, Yamakami Y and Fujii M. IDENTIFICATION OF MANUBRIUM PIGMENTS IN TWO SPECIES OF Turritopsis MEDUSAE (CNIDARIA, HYDROZOA) IN JAPAN. Kurosio Biosphere. 2018, 14, 10-16

2017年

  1. Taoka M, Fujii M, Tsuchiya M, Uekita T, Ichimura T. A sensitive microbread-based organic media assisted method for proteomics sample preparation from dilute and denaturing solutions. ACS Appl Mater Interfaces. 2017, 9(49), 42661-42667.
  2. Mahmud S, Haque S, Sarker D, Ayusawa D, Hossain MN. Disinfection of supplied drinking water by poly-herbal preparation. BJST. 2017 Dec
  3. Takauji Y, Kudo I, En A, Matsuo R, Hossain MN, Nakabayashi K, Miki K, Fujii M and Ayusawa D. GNG11 (G-protein sununit 11) suppresses cell growth with induction of reactive oxygen species and abnormal nuclear morphology in human SUSM-1 cells. Biochem Cell Biol. 2017 95:517-523
  4. Matsuo R, Mizobuchi S, Nakashima M, Miki K, Ayusawa D and Fujii M. Central roles of iron in the regulation of oxidative stress in the yeast Saccharomyces cerevisiae. Currt Genet. 2017, 63:895-907.

2016年

  1. Awaya A. Suppressive influence of seasonal influenza epidemic on Kawasaki disease onset. Jpn. J. Clin. Immunol. 2016, 6, 528-537.
  2. Hossain MN, Azim DA, Ayusawa D, Sarkar AK and Ferdous T. Evaluation of poly herbal formulation in the management of Acne and Melasma. Int J Ayu Pharm Chem. 2016 Nov 16.
  3. Takauji Y, Morino K, Miki K, Hossain MN, Ayusawa D and Fujii M. Chyawanprash, a formulation of traditional Ayurvedic medicine, shows a protective effect on skin photoaging in hairless mice. J Integrative Medicine. 2016, 14,473-479.
  4. Takauji Y, Miki K, Mita J, Hossain MN, Yamauchi M, Kioi M, Ayusawa D and Fujii M. Triphala, a formulation of traditional Ayurvedic medicine, shows protective effect against X-radiation in HeLa cells. J Biosci. 2016, 41, 569-575.
  5. Kudo I, Nozawa M, Miki K, Takauji Y, En A, Fujii M and Ayusawa D. Dual roles of ERK1/2 in cellular senescence induced by excess thymidine in HeLa cells. Exp Cell Res. 2016, 346:216-223.
  6. Takauji Y, En A, Miki K, Ayusawa D and Fujii M. Combinatorial effects of continuous protein synthesis, ERK-signaling, and reactive oxygen species on induction of cellular senescence. Exp Cell Res. 2016, 345:239-46.
  7. Rumi Arai, Atsuki En, Ryo Ukekawa, Kensuke Miki, Michihiko Fujii, Dai Ayusawa. Aberrant localization of lamin B receptor (LBR) in cellular senescence in human cells. Biochem Biophys Res Commun 2016, 473:1078-83.
  8. Takauji Y, Wada T, Takeda A, Kudoh I, Miki K, Fujii M and Ayusawa D. Restriction of protein synthesis abolishes senescence features at cellularand organismal levels. Sci Rep. 2016, 6:18722.
  9. Yamakami Y, Yonekura R, Matsumoto Y, Takauji Y, Miki K, Fujii M and Ayusawa D. High concentrations of NaCl induce cell swelling leading to senescence in human cells. Mol Cell Biochem. 2016, 411:117-25.

2015年

  1. Sattya Narayan Talukdar, Dai Ayusawa, Md. Rasel Al Mahmud, Mohammad Nazir Hossain. D4476, a cell-permeant inhibitor of CK1, potentiates the action of Bromodeoxyuridine in inducing senescence in HeLa cells. Biojournal of Science and Technology. 2015 jan 15

2014年

  1. Yamakami Y, Miki K, Yonekura R, Kudo I, Fujii M, Ayusawa D. Molecular basis for premature senescence induced by surfactants in normal human cells. Biosci Biotechnol Biochem. 2014,78(12):2022-9.
  2. Awaya A, Nishimura C. A combination of cross correlation andtrend analyses reveals that Kawasaki disease is a pollen-induced delayed-typehyper-sensitivity disease. Int J Environ Res Public Health. 2014 Mar 4;11(3):2628-41.

2012年

  1. Ueno S, Yasutake K, Tohyama D, Fujimori T, Ayusawa D, Fujii M. Systematic screen for genes involved in the regulation of oxidative stress in the nematode Caenorhabditis elegans. Biochem Biophys Res Commun. 2012,420(3):552-7.
  2. Kobayashi Y, Lee SS, Arai R, Miki K, Fujii M, Ayusawa D. ERK1/2 mediates unbalanced growth leading to senescence induced by excess thymidine in human cells.Biochem Biophys Res Commun. 2012,425(4):897-901.
  3. Akira Awaya and Koji Murayama. Positive correlation between Japanese cedar pollen numbers and the development of Kawasaki disease.The Open Allergy Journal, 2012, 5, 1-10

2011年

  1. Fujii M, Shikatani K, Ogura K, Goshima Y, Ayusawa D. Mutation in a mitochondrial ribosomal protein causes increased sensitivity to oxygen with decreased longevity in the nematode Caenorhabditis elegans. Genes Cells. 2011,16(1):69-79.
  2. Yamakami Y, Miki K, Endoh M, Yonekura R, Ukekawa R, Kobayashi Y, Fujii M, Ayusawa D. Sublethal doses of surfactants induce premature senescence in normal human skin cells. Biosci Biotechnol Biochem. 2011,75(7):1395-8.
  3. Takayama S, Fujii M, Nakagawa Y, Miki K, Ayusawa D. N-terminal short fragment of TUP1 confers resistance to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2011,411(1):25-31.
  4. Fujii M, Yasuda K, Hartman PS, Ayusawa D, Ishii N. A mutation in a mitochondrial dehydrogenase/reductase gene causes an increased sensitivity to oxidative stress and mitochondrial defects in the nematode Caenorhabditis elegans.Genes Cells. 2011,16(10):1022-34.

2010年

  1. Fujii M,Miki K,Takayama S,Ayusawa D. Identification of genes that affect sensitivity to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae. Mol Genet Genomics. 2010,283(5):461-8.
  2. Miki K, Shimizu M, Fujii M, Takayama S, Hossain MN, Ayusawa D. 5-bromodeoxyuridine induces transcription of repressed genes with disruption of nucleosome positioning. FEBS J. 2010,277(21):4539-48.

2009年

  1. Fujii M, Adachi N, Shikatani K, Ayusawa D. [FeFe]-hydrogenase-like gene is involved in the regulation of sensitivity to oxygen in yeast and nematode. Genes Cells. 2009 ,14(4):457-68.
  2. Endoh M, Kobayashi Y, Yamakami Y, Yonekura R, Fujii M, Ayusawa D. Coordinate expression of the human pregnancy-specific glycoprotein gene family during induced and replicative senescence. Biogerontology. 2009,10(2):213-21.

2008年

  1. Kobayashi Y, Sakemura R, Kumagai A, Sumikawa E, Fujii M, Ayusawa D.Nuclear swelling occurs during premature senescence mediated by MAP kinases in nomal human fibroblasts. Biosci Biotechnol Biochem. 2008, 72(4): 1122-5.
  2. Miki K, Shimizu M, Fujii M, Hossain MN, Ayusawa D. 5-Bromouracil disrupts nucleosome positioning by inducing A-form-like DNA conformation in yeast cells. Biochem Biophys Res Commun. 2008, 368(3): 662-9.
  3. Kurosawa A, Koyama H, Takayama S, Miki K, Ayusawa D, Fujii M, Iiizumi S, Adachi N. The requirement of Artemis in double strand break repair depends on the type of DNA damage. DNA Cell Biol. 2008, 27(1): 55-61.

2007年

  1. Takayama S, Fujii M, Kurosawa A, Adachi N, Ayusawa D. Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae. Curr Genet. 2007, 52(5-6): 203-11.
  2. Arivazhagan P, Ayusawa D. Cardiolipin activates MAP kinases during premature senescence in normal human fibroblasts. Biogerontology. 2007, 8(6): 621-6.
  3. Endoh M, Miki K, Hossain MN, Fujii M, Ayusawa D. 5-Bromodeoxyuridine increases transient expression of ectopic genes in human cells. Biosci Biotechnol Biochem. 2007, 71(4):1098-102.
  4. Hossain MN, Fujii M, Miki K, Endoh M, Ayusawa D. Downregulation of hnRNP C1/C2 by siRNA sensitizes HeLa cells to various stresses. Mol Cell Biochem. 2007, 296(1-2): 151-7.
  5. Ukekawa R, Miki K, Fujii M, Hirano H, Ayusawa D. Accumulation of multiple forms of lamin A with down-regulation of FACE-1suppresses growth in senescent human cells.Genes Cells. 2007, 12(3): 397-406.

2006年

  1. Arivazhagan P, Ayusawa D, Panneerselvam C. Protective efficacy of alpha-lipoic acid on acetylcholinesterase activity in aged rat brain regions. Rejuvenation Res. 2006, 9 (2): 198-201.
  2. Hossain MN, Sakemura R, Fujii M, Ayusawa D. G-protein gamma subunit GNG11 strongly regulates cellular senescence. Biochem Biophys Res Commun. 2006, 351(3): 645-50.
  3. Fujii M, Kawai Y, Endoh M, Hossain MN, Nakabayashi K, Ayusawa D. Expression of RAB27B is up-regulated in senescent human cells. Mech Ageing Dev. 2006, 127(7): 639-42.

2005年

  1. Fujii M, Tanaka N, Miki K, Hossain MN, Endoh M, Ayusawa D. Uncoupling of longevity and paraquat resistance in mutants of the nematode Caenorhabditis elegans. Biosci Biotechnol Biochem. 2005, 69(10):2015-8.
  2. Sumikawa E, Matsumoto Y, Sakemura R, Fujii M, Ayusawa D. Prolonged unbalanced growth induces cellular senescence markers linked with mechano transduction in normal and tumor cells. Biochem Biophys Res Commun. 2005, 335(2): 558-65.
  3. Minagawa S, Nakabayashi K, Fujii M, Scherer SW, Ayusawa D. Early BrdU-responsive genes constitute a novel class of senescence-associated genes in human cells. Exp Cell Res. 2005, 304(2): 552-8.

2004年

  1. Ukekawa R, Maegawa N, Mizutani E, Fujii M, Ayusawa D. Proteasome inhibitors induce changes in chromatin structure characteristic of senescent human fibroblasts. Biosci Biotechnol Biochem. 2004, 68(11): 2395-7.
  2. Arivazhagan P, Mizutani E, Fujii M, Ayusawa D. Cardiolipin induces premature senescence in normal human fibroblasts. Biochem Biophys Res Commun. 2004, 323(3): 739-42.
  3. Minagawa S, Nakabayashi K, Fujii M, Scherer SW, Ayusawa D. Functional and chromosomal clustering of genes responsive to 5-bromodeoxyuridine in human cells. Exp Gerontol. 2004, 39(7): 1069-78.
  4. Joguchi A, Fujii M, Ayusawa D. Increased catalase activity in mouse cell mutants resistant to paraquat. Biogerontology. 2004, 5(3): 193-200.
  5. Satou W, Suzuki T, Noguchi T, Ogino H, Fujii M, Ayusawa D. AT-hook proteins stimulate induction of senescence markers triggered by 5-bromodeoxyuridine in mammalian cells. Exp Gerontol. 2004, 39(2): 173-9.
  6. Satou W, Tanimoto H, Ukekawa R, Fujii M, Ayusawa D. Amplification of nuclear aldolase A in mouse cell mutants resistant to Hoechst33342. Biochem Biophys Res Commun. 2004, 315(4): 845-9.
  7. Fujii M, Matsumoto Y, Tanaka N, Miki K, Suzuki T, Ishii N, Ayusawa D. Mutations in chemosensory cilia cause resistance to paraquat in nematode Caenorhabditis elegans. J Biol Chem. 2004, 279(19): 20277-82.
  8. Awaya A, Sahashi N. The etiology of Kawasaki disease: Does intense release of pollen induce pollinosis in constitutionally allergic adults, while constitutionally allergic infants develop Kawasaki disease? Biomedicine & Pharmacotherapy, 2004 Mar;58(2):136-40.

2003年

  1. Awaya A, Watanabe K, Kato S. Individuals exhibiting conspicuous nevi (lentigo　simplex) are resistant to allergic rhinitis/conjunctivitis (pollinosis), but those who do not show increased susceptibility to pollinosis. Microbiol Immunol. 2003;47(1):101-3.

2002年

  1. Lettice LA, Horikoshi T, Heaney SJ, van Baren MJ, van der Linde HC,Breedveld GJ, Joosse M, Akarsu N, Oostra BA, Endo N, Shibata M, Suzuki M, Takahashi E, Shinka T, Nakahori Y, Ayusawa D, Nakabayashi K, Scherer SW, Heutink P, Hill RE, Noji S. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly. Proc Natl Acad Sci U S A. 2002, 99:7548-53.
  2. Ogino H, Satou W, Fujii M, Suzuki T, He Y, Michishita E, Ayusawa D. The human MYOD1 transgene is suppressed by 5-bromodeoxyuridine in mouse myoblasts. J Biochem (Tokyo). 2002, 132(6): 953-9.
  3. Suzuki T, Fujii M, Ayusawa D. Demethylation of classical satellite 2 and 3 DNA with chromosomal instability in senescent human fibroblasts. Exp Gerontol. 2002, 37(8-9): 1005-14.
  4. Michishita E, Kurahashi T, Suzuki T, Fukuda M, Fujii M, Hirano H, Ayusawa D. Changes in nuclear matrix proteins during the senescence-like phenomenon induced by 5-chlorodeoxyuridine in HeLa cells. Exp Gerontol. 2002, 37(7): 885-90.
  5. Joguchi A, Otsuka I, Minagawa S, Suzuki T, Fujii M, Ayusawa D. Overexpression of VDUP1 mRNA sensitizes HeLa cells to paraquat. Biochem Biophys Res Commun. 2002, 293(1): 293-7.
  6. Fujii M, Ito H, Hasegawa T, Suzuki T, Adachi N, Ayusawa D. 5-Bromo-2'-deoxyuridine efficiently suppresses division potential of the yeast Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2002, 66(4): 906-9.
  7. Michishita E, Matsumura N, Kurahashi T, Suzuki T, Ogino H, Fujii M, Ayusawa D. 5-Halogenated thymidine analogues induce a senescence-like phenomenon in HeLa cells. Biosci Biotechnol Biochem. 2002, 66(4): 877-9.
  8. Suzuki T, Michishita E, Ogino H, Fujii M, Ayusawa D. Synergistic induction of the senescence-associated genes by 5-bromodeoxyuridine and AT-binding ligands in HeLa cells. Exp Cell Res. 2002, 276(2): 174-84.
  9. Ogino H, Fujii M, Satou W, Suzuki T, Michishita E, Ayusawa D. Binding of 5-bromouracil-containing S/MAR DNA to the nuclear matrix. DNA Res. 2002, 9(1): 25-9.

2001年

  1. Suzuki T, Yaginuma M, Oishi T, Michishita E, Ogino H, Fujii M, Ayusawa D. 5-Bromodeoxyuridine suppresses position effect variegation of transgenes in HeLa cells. Exp Cell Res. 2001, 266(1): 53-63.
  2. Suzuki T, Minagawa S, Michishita E, Ogino H, Fujii M, Mitsui Y, Ayusawa D. Induction of senescence-associated genes by 5-bromodeoxyuridine in HeLa cells. Exp Gerontol. 2001, 36(3): 465-74.
  3. Sado K, Ayusawa D, Enomoto A, Suganuma T, Oshimura M, Sato K, Koyama H. Identification of a mutated DNA ligase IV gene in the X-ray-hypersensitive mutant SX10 of mouse FM3A cells. J Biol Chem. 2001 276:9742-8.

2000年

  1. Ishiia H, Sasaki Y, Goshima Y, Kanai Y, Endou H, Ayusawa D, Ono H, Miyamae T, Misu Y. Involvement of rBAT in Na(+)-dependent and -independent transport of the neurotransmitter candidate L-DOPA in Xenopus laevis oocytes injected with rabbit small intestinal epithelium poly A(+) RNA. Biochim Biophys Acta. 2000 1466:61-70.
  2. Fujiwara J, Fujii M, Shimoda M, Ayusawa D. Identification of the UV-responsive sequence in the human tissue plasminogenactivator gene. Biosci Biotechnol Biochem. 2000, 64(5):1084-7.

1999年

  1. Michishita E, Nakabayashi K, Suzuki T, Kaul SC, Ogino H, Fujii M, Mitsui Y, Ayusawa D. 5-Bromodeoxyuridine induces senescence-like phenomena in mammalian cells regardless of cell type or species. J Biochem (Tokyo). 1999, 126(6): 1052-9.
  2. Fujii M, Joguchi A, Ogino H, Nakabayashi K, Ayusawa D. Dephosphorylation of specific proteins during induction of senescence in immortal human fibroblasts expressing thermolabile SV40 T antigen. Biosci Biotechnol Biochem. 1999, 63(6): 1116-8.
  3. Fujii M, Ide A, Nakabayashi K, Joguchi A, Ogino H, Ayusawa D. The introduction of dominant-negative p53 mutants suppresses temperature shift-induced senescence in immortal human fibroblasts expressing a thermolabile SV40 large T antigen. J Biochem (Tokyo). 1999, 125(3): 531-6.

1998年

  1. Michishita E, Nakabayashi K, Ogino H, Suzuki T, Fujii M, Ayusawa D. DNA topoisomerase inhibitors induce reversible senescence in normal human fibroblasts. Biochem Biophys Res Commun. 1998, 253(3): 667-71.
  2. Ogino H, Nakabayashi K, Michishita E, Scherer SW, Fujii M, Suzuki T, Ayusawa D. Clustering of human chromosome fragments on the mouse genome by chromosome-mediated gene transfer. DNA Res. 1998, 5(4) :247-50.
  3. Ide A, Fujii M, Nakababashi K, Ayusawa D. Suppression of senescence in normal human fibroblasts by introduction of dominant-negative p53 mutants or human papilloma virus type 16 E6 protein. Biosci Biotechnol Biochem. 1998, 62(7): 1458-60.
  4. Ishii N, Fujii M, Hartman PS, Tsuda M, Yasuda K, Senoo-Matsuda N, Yanase S, Ayusawa D, Suzuki K. A mutation in succinate dehydrogenase cytochrome b causes oxidative stress and ageing in nematodes. Nature. 1998, 394(6694): 694-7.
  5. Ogino H, Nakabayashi K, Suzuki M, Takahashi E, Fujii M, Suzuki T, Ayusawa D. Release of telomeric DNA from chromosomes in immortal human cells lacking telomerase activity. Biochem Biophys Res Commun. 1998, 248(2): 223-7.
  6. Fujii M, Ishii N, Joguchi A, Yasuda K, Ayusawa D. A novel superoxide dismutase gene encoding membrane-bound and extracellular isoforms by alternative splicing in Caenorhabditis elegans. DNA Res. 1998, 5(1): 25-30.

1997年

  1. Nakabayashi K, Ogata T, Fujii M, Tahara H, Ide T, Wadhwa R, Kaul SC, Mitsui Y, Ayusawa D. Decrease in amplified telomeric sequences and induction of senescence markers by introduction of human chromosome 7 or its segments in SUSM-1. Exp Cell Res. 1997, 235(2): 345-53.