{"id":89,"date":"2024-07-06T17:33:12","date_gmt":"2024-07-06T17:33:12","guid":{"rendered":"https:\/\/www-user.yokohama-cu.ac.jp\/~aritak7225\/wp\/?page_id=89"},"modified":"2024-08-30T12:19:49","modified_gmt":"2024-08-30T12:19:49","slug":"facility","status":"publish","type":"page","link":"https:\/\/www-user.yokohama-cu.ac.jp\/~aritak7225\/facility\/","title":{"rendered":"\u7814\u7a76\u5ba4\u306e\u69d8\u5b50\u30fb\u7814\u7a76\u8a2d\u5099"},"content":{"rendered":"\n

\u7814\u7a76\u5ba4<\/h4>\n\n\n\n
\"\"<\/figure>
\n

\u5c45\u5ba4<\/strong><\/p>\n\n\n\n

PC\u3068\u30c7\u30b9\u30af\u304c\u7528\u610f\u3055\u308c\u307e\u3059\u3002\u8ad6\u6587\u57f7\u7b46\u3001\u8ad6\u6587\u691c\u7d22\u3001\u7814\u7a76\u767a\u8868\u8cc7\u6599\u306e\u307e\u3068\u3081\u306a\u3069\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u5b9f\u9a13\u5ba4<\/strong><\/p>\n\n\n\n

\u69cb\u9020\u89e3\u6790\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u8a66\u6599\u306e\u8abf\u88fd\u3001Buffer\u306a\u3069\u306e\u8a66\u85ac\u8abf\u88fd\u3001\u96fb\u6c17\u6cf3\u52d5\u306a\u3069\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure>
\n

\u56fd\u969b\u4ea4\u6d41<\/strong><\/p>\n\n\n\n

\u5171\u540c\u7814\u7a76\u8005\u306ePierre-Antoine Defossez\u5148\u751f (Paris Diderot University) \u304c2019\u5e7412\u6708\u306b\u6765\u65e5\u3057\u3001Mini Lab Seminar\u3092\u884c\u3044\u307e\u3057\u305f\u3002
Ferry Mol Cell<\/em> 2017, Kori Structure<\/em> 2019, Kori BMC<\/em> 2021, Kikuchi Nature Communications<\/em> 2022 \u3067\u5171\u8457\u8ad6\u6587\u3092\u767a\u8868\u3057\u3001\u73fe\u5728\u3082\u5171\u540c\u7814\u7a76\u304c\u7d9a\u3044\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u5b66\u751f\u306e\u6d3b\u8e8d<\/strong><\/p>\n\n\n\n

\u56fd\u5185\u5916\u306e\u5b66\u4f1a\u3067\u7a4d\u6975\u7684\u306b\u767a\u8868\u3057\u3066\u307e\u3059\u3002\u5b66\u751f\u3082\u53e3\u982d\u767a\u8868\u3057\u3066\u307e\u3059\u3002
\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\u7814\u7a76\u4f1a\u3001\u65e5\u672c\u5206\u5b50\u751f\u7269\u5b66\u4f1a\u3001\u86cb\u767d\u8cea\u79d1\u5b66\u4f1a\u3001\u65e5\u672c\u751f\u5316\u5b66\u4f1a\u306a\u3069\u3002
\u69d8\u3005\u306a\u5b66\u4f1a\u3067\u6570\u591a\u304f\u306e\u5b66\u751f\u304c\u512a\u79c0\u767a\u8868\u8cde\u3092\u7372\u5f97\u3057\u3066\u307e\u3059\u3002<\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n

\u5b9f\u9a13\u8a2d\u5099<\/h4>\n\n\n\n
\"\"<\/figure>
\n

\u7cbe\u88fd\uff1aAKTA go\uff08Cytiva\u793e 3\u53f0\u4fdd\u6709<\/strong>\uff09<\/p>\n\n\n\n

\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u306e\u88c5\u7f6e\u3067\u3059\u3002\u7cbe\u88fd\u306e\u4e2d\u76e4\u304b\u3089\u5f8c\u534a\u3067\u4f7f\u7528\u3057\u307e\u3059\u3002\u30a4\u30aa\u30f3\u4ea4\u63db\u30ab\u30e9\u30e0\u3001\u758e\u6c34\u6027\u30ab\u30e9\u30e0\u3001\u30b0\u30eb\u308d\u904e\u30ab\u30e9\u30e0\u306a\u3069\u3092\u88c5\u7f6e\u306b\u3064\u306a\u3052\u3066\u7cbe\u88fd\u3057\u307e\u3059\u3002\u30a8\u30a2\u30bb\u30f3\u30b5\u2015\u304c\u3064\u3044\u3066\u304a\u308a\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u30ab\u30e9\u30e0\u3078\u306e\u30ed\u30fc\u30c9\u3001\u30ab\u30e9\u30e0\u306e\u6d17\u6d44\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u6eb6\u51fa\u306e\u4e00\u9023\u306e\u6d41\u308c\u3092\u81ea\u52d5\u3067\u3067\u304d\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u306f\u65e5\u5e38\u7684\u306b\u884c\u3046\u306e\u3067\u3001\u5e38\u306b\u7a3c\u50cd\u3057\u3066\u3044\u308b\u88c5\u7f6e\u3067\u3059\u3002
\u203b\u3059\u3079\u3066\u306e\u8ad6\u6587\u3067\u4f7f\u7528<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u7cbe\u88fd\uff1aAKTA pure\uff08Cytiva\u793e 1\u53f0\u4fdd\u6709\uff09<\/strong><\/p>\n\n\n\n

\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6700\u7d42\u7cbe\u88fd\u3084\u9ad8\u5206\u96e2 (\u5f37\u30a4\u30aa\u30f3\u30ab\u30e9\u30e0\u3092\u4f7f\u7528) \u3092\u76ee\u6307\u3059\u6642\u306b\u4f7f\u3044\u307e\u3059\u3002\u5206\u96e2\u80fd\u306e\u9ad8\u3044\u30b2\u30eb\u308d\u904e\u30ab\u30e9\u30e0\u3067\u3042\u308bINCREASE\u30ab\u30e9\u30e0 (Cytiva\u793e) \u3092\u7528\u3044\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6027\u72b6\u89e3\u6790\u306b\u3082\u4f7f\u3044\u307e\u3059\u3002
\u203b\u3059\u3079\u3066\u306e\u8ad6\u6587\u3067\u4f7f\u7528<\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure>
\n

\u7d50\u6676\u5316\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u5fae\u91cf\u7d50\u6676\u5316\u88c5\u7f6e Crystal Gryphon\uff08Art Robbins Instruments\u793e\uff09<\/strong><\/strong><\/p>\n\n\n\n

\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\u3068\u7d50\u6676\u5316buffer\u3092\u6df7\u305c\u5408\u308f\u305b\u3066\u30b7\u30c3\u30c6\u30a3\u30f3\u30b0\u30c9\u30ed\u30c3\u30d7\u84b8\u6c17\u62e1\u6563\u6cd5\u3067\u7d50\u6676\u5316\u3057\u307e\u3059\u3002\u6700\u5c0f\u3067200 nl\u306e\u7d50\u6676\u5316\u30c9\u30ed\u30c3\u30d7\u304c\u4f5c\u6210\u3067\u304d\u307e\u3059\u3002\u7d50\u6676\u5316\u306e\u521d\u671f\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306b\u4f7f\u3044\u307e\u3059\u3002
Ishiyama Mol Cell<\/em> 2017, Kori Structure<\/em> 2019, Jimenji BBRC<\/em> 2019, Kori BMC<\/em> 2021. Shiraishi Commun Biol<\/em> 2024.<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u7cbe\u88fd\uff1aHPLC\u88c5\u7f6e\uff08SHIMAZU\u793e\uff09<\/strong><\/strong><\/strong><\/p>\n\n\n\n

\u30da\u30d7\u30c1\u30c9\u7cbe\u88fd\u306b\u4f7f\u3044\u307e\u3059\u3002\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u5929\u7136\u5909\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b\u30d2\u30b9\u30c8\u30f3H3\u306eN\u672b\u7aef\u9818\u57df\u3084\u8907\u88fd\u56e0\u5b50PAF15\u3092\u5927\u8178\u83cc\u3067\u767a\u73fe\u3055\u305b\u3001\u6700\u7d42\u7cbe\u88fd\u306bHPLC\u3092\u7528\u3044\u307e\u3059\u3002
Hata NAR<\/em> 2022.<\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure>
\n

\u89e3\u6790\uff1aCFX Connect\uff08Bio-Rad\u793e\uff09<\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u71b1\u5909\u6027\u6e29\u5ea6\u3092\u89b3\u6e2c\u3057\u3001\u5b89\u5b9a\u6027\u3084\u5316\u5408\u7269\u3068\u306e\u7d50\u5408\u306e\u8a55\u4fa1\u306b\u4f7f\u3044\u307e\u3059\u3002
Kori JMB<\/em> 2020\u306eFig 5\u306e\u5b9f\u9a13\u3067\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002Kori BMC<\/em> 2021.<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u89e3\u6790\uff1aSynergy2\uff08Biotek\u793e\uff09<\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

384\u30d7\u30ec\u30fc\u30c8\u304c\u8aad\u307f\u53d6\u308c\u308b\u86cd\u5149\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u3067\u3059\u3002\u86cd\u5149\u5f37\u5ea6\u306e\u89b3\u6e2c\u304b\u3089\u9175\u7d20\u53cd\u5fdc\u306e\u8ffd\u8de1\u304c\u53ef\u80fd\u3067\u3059\u3002\u307e\u305f\u3001\u504f\u5149\u677f\u3092\u5165\u308c\u3066\u3044\u308b\u306e\u3067\u3001\u86cd\u5149\u504f\u5149\u89e3\u6d88\u6cd5\u306b\u3088\u308b\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u304c\u4ed8\u3044\u305f\u30da\u30d7\u30c1\u30c9\u3084DNA\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u76f8\u4e92\u4f5c\u7528\u3092\u5b9a\u91cf\u7684\u306b\u89e3\u6790\u3067\u304d\u307e\u3059\u3002
Ferry Mol Cell<\/em> 2017, Kori JMB<\/em> 2020. Kori BMC<\/em> 2021\u3067\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure>
\n

\u57f9\u990a\uff1a\u6606\u866b\u7d30\u80de\u7528\u57f9\u990a\u88c5\u7f6e<\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

Sf9\u7d30\u80de\u306e\u57f9\u990a\u306b\u4f7f\u3044\u307e\u3059\u3002\u57f9\u990a\u6e29\u5ea6\u306f27\u2103\u3067\u3059\u3002
\u4e0a\u306e\u6bb5\u306f6\u7a74\u30d7\u30ec\u30fc\u30c8\u306a\u3069\u3092\u7528\u3044\u3066\u3001\u7d30\u80de\u3092\u63a5\u7740\u3055\u305b\u3066\u57f9\u990a\u3057\u307e\u3059\u3002\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u5e45\u6642\u306b\u4f7f\u7528\u3057\u307e\u3059\u3002
\u4e0b\u306e\u6bb5\u306e\u57f9\u990a\u5668\u3067\u306f\u3001\u30d5\u30e9\u30b9\u30b3\u306b\u57f9\u5730\u3092\u5165\u308c\u3001\u7d30\u80de\u3092\u6d6e\u904a\u3055\u305b\u3066\u57f9\u990a\u3057\u307e\u3059\u3002\u4e3b\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3055\u305b\u308b\u6642\u306b\u4f7f\u3044\u307e\u3059\u3002
\u5927\u8178\u83cc\u3067\u306f\u767a\u73fe\u3057\u306a\u3044\u5206\u5b50\u91cf\u306e\u5927\u304d\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u3092Sf9\u7d30\u80de\u3067\u767a\u73fe\u3055\u305b\u307e\u3059\u3002<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u57f9\u990a\uff1aINNOVA\uff08\u30a8\u30c3\u30da\u30f3\u30c9\u30eb\u30d5\u793e\uff09<\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

\u5927\u8178\u83cc\u306e\u5927\u91cf\u57f9\u990a\u306b\u4f7f\u3046\u57f9\u990a\u6a5f\u3067\u3059\u30022\u53f0\u91cd\u306d\u3066\u8a2d\u7f6e\u3057\u3066\u3044\u307e\u3059\u30021\u53f0\u30675L\u30d5\u30e9\u30b9\u30b3 (\u57f9\u57302L) \u304c4\u672c\u5165\u308a\u307e\u3059\u3002
\u57f9\u990a\u6e29\u5ea6\u306f\u767a\u73fe\u3055\u305b\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7a2e\u985e\u306b\u3088\u3063\u306615\u2103\uff5e37\u2103\u306e\u9593\u3067\u884c\u3044\u307e\u3059\u3002
\u203b\u3059\u3079\u3066\u306e\u8ad6\u6587\u3067\u4f7f\u7528<\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure>
\n

\u89e3\u6790\uff1aChemiDoc\uff08Bio-Rad\u793e\uff09<\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

\u96fb\u6c17\u6cf3\u52d5\u30b2\u30eb\u306e\u30a4\u30e1\u30fc\u30b8\u30e3\u30fc\u3067\u3059\u3002CBB\u3067\u67d3\u8272\u3057\u305fSDS-PAGE\u30b2\u30eb\u306e\u64ae\u5f71\u3001\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u691c\u51fa (\u5316\u5b66\u767a\u5149)\u3001\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u304b\u3089\u306e\u767a\u5149\u306e\u691c\u51fa\u304c\u3067\u304d\u307e\u3059\u3002
\u203b\u3059\u3079\u3066\u306e\u8ad6\u6587\u3067\u4f7f\u7528<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u907a\u4f1d\u5b50\u5b9f\u9a13\uff1a\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc<\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

PCR\u306b\u3088\u308b\u76ee\u7684\u306eDNA\u306e\u5897\u5e45\u306b\u4f7f\u3044\u307e\u3059\u3002TAKARA\u793e\u3068BIORAD\u793e\u306e2\u53f0\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u3069\u3061\u3089\u3082\u30bf\u30c3\u30c1\u30d1\u30cd\u30eb\u3067\u6271\u3044\u3084\u3059\u3044\u3067\u3059\u3002
\u203b\u3059\u3079\u3066\u306e\u8ad6\u6587\u3067\u4f7f\u7528<\/p>\n<\/div>

\"\"<\/figure><\/div>\n\n\n\n
\"\"<\/figure>
\n

\u4fdd\u5b58\uff1a\u6975\u4f4e\u6e29\u30d5\u30ea\u30fc\u30b6\u30fc<\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

\u5de6\uff1a-80\u2103\u306b\u8a2d\u5b9a\u3055\u308c\u305f\u51b7\u51cd\u5eab\u3067\u3059\u3002\u7cbe\u88fd\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u5927\u8178\u83cc\u306a\u3069\u306e\u751f\u4f53\u8a66\u6599\u306e\u4fdd\u5b58\u3092\u3057\u307e\u3059\u3002
\u53f3\uff1a-20\u2103\u306b\u8a2d\u5b9a\u3055\u308c\u305f\u51b7\u51cd\u5eab\u3067\u3059\u3002\u5408\u6210\u30da\u30d7\u30c1\u30c9\u3001\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3001\u8a66\u85ac\u306a\u3069\u3092\u4fdd\u5b58\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div><\/div>\n\n\n\n

\n

\u4fdd\u5b58\uff1a\u7d50\u6676\u5316\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc<\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u6676\u3092\u6210\u9577\u3055\u305b\u308b\u305f\u3081\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u3067\u3059\u300220\u2103\u306b\u7ba1\u7406\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div>

\"\"<\/figure><\/div>\n","protected":false},"excerpt":{"rendered":"

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