DNA supercoiling factor (SCF)

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DNA supercoiling factor (SCF) is capable of generating negative supercoils into a relaxed DNA in conjunction with eukaryotic DNA topoisomerase II. SCF has 322 residues and contains six EF-hands, five of which are predicted to bind calcium. SCF requires ~10 _M Ca2+ for activity. This is inferred to reflect an in vivo response to a calcium signal [29]. Several domains of SCF and RTC are most closely related; however, there is no apparent relation in their functions nor homology in the domains at their N-termini.