Ras guanyl nucleotide-releasing protein (GRP)

entry list
alignment
Ras guanyl nucleotide-releasing protein (GRP) has 795 residues and contains a homolog of a REM domain, a CDC25 domain, two EF-hands, and a diacylglycerol binding domain [83]. The two EF-hand loops are canonical and are expected to bind calcium. Helices E1 and F2 appear conventional. However, there are only 15 residues for helices F1 and E2. One or both of these helices is truncated and the relationship between the two EF-hands is unclear. Rat fibroblasts expressing the RasGRP encoding gene vector are transformed. Addition of a DAG analog causes sustained activation of Ras-Erk signaling and changes in cell morphology.