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table4 |
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Name |
Protease/chemicals |
Sites/sizes |
Properties/methods |
Refs |
|
1 |
Calmodulin |
Trypsin |
|
Sequence determination |
|
|
|
|
Chymotrypsin |
|
Sequence determination |
|
|
|
|
BrCN |
|
Sequence determination |
|
|
|
|
Trypsin |
1-77, 78-148, 1-106, 107-148 |
Preparation and analysis |
3164, 2326, 2779 |
|
|
|
Trypsin |
1-77, 78-148 (+Ca), 1-106, 1-90, 107-148 (-Ca) |
Enzyme activation |
5772, 5642, 6074, 3609, 4845 |
|
|
|
Trypsin |
78-148 |
SAXA |
6842 |
|
|
|
Trypsin |
1-77, 78-148 |
Microcalorimetry |
426 |
|
|
|
Trypsin |
1-77, 78-148 |
Stopped-flow fluorescence |
369, 444 |
|
|
|
Trypsin |
1-77, 78-148 |
Peptide binding |
5924, 5981, 5993, 5734 |
|
|
|
Trypsin |
78-148 |
NMR |
2308, 2585, 1330 |
|
|
|
Trypsin |
1-75, 78-148 |
NMR |
331, 460, 1331, 290 |
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|
Trypsin |
1-75, 78-148 |
Metal ion binding |
371, 391 |
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|
Thrombin |
1-106, 107-148 |
NMR |
239 |
|
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CAM endoproteinase |
Lys75 |
|
909 |
|
|
|
Fungal protease |
|
|
2953 |
|
|
|
Bacterial proteases |
|
|
2953 |
|
|
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?? |
72-148 |
|
4220 |
|
2 |
Troponin C |
Lysylendopeptidase |
|
Sequence determination |
|
|
|
|
Staphylococcus aureus V8 protease |
|
Sequence determination |
|
|
|
|
BrCN |
|
Sequence determination |
|
|
|
|
Trypsin |
|
Sequence determination |
|
|
|
|
Trypsin |
9-84, 89-159 |
Ca-binding, NMR |
3023, 2052 |
|
|
|
Thrombin |
1-120, 121-159 |
Ca-binding, NMR |
3023, 2052 |
|
|
|
BrCN |
46-77, 84-135 |
Ca-binding, NMR |
3023, 2052 |
|
3 |
Essential light chain |
Trypsin |
|
Sequence determination |
|
|
|
|
Chymotrypsin |
|
Sequence determination |
|
|
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|
BrCN |
|
Sequence determination |
|
|
4 |
Regulatory light chain |
Trypsin |
|
Sequence determination |
|
|
|
|
Chymotrypsin |
|
Sequence determination |
|
|
|
|
Pepsin |
|
Sequence determination |
|
|
5 |
Troponin C, nonvertebrate |
Lysylendopeptidase |
|
Sequence determination |
|
|
|
|
S. aureus V8 protease |
|
Sequence determination |
|
|
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|
BrCN |
|
Sequence determination |
|
|
7 |
Squidulin |
Endoproteinase-Arg C |
|
Sequence determination |
|
|
|
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Trypsin |
|
Sequence determination |
|
|
|
|
BrCN |
|
Sequence determination |
|
|
|
|
Hydroxylamine |
|
Sequence determination |
|
|
|
|
Carboxypeptidase Y |
|
Sequence determination |
|
|
11 |
Calcineurin B |
Trypsin |
|
Sequence determination |
|
|
|
|
S. aureus V8 protease |
|
Sequence determination |
|
|
|
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Lysine-specific protease |
|
Sequence determination |
|
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BrCN |
|
Sequence determination |
|
|
20 |
Calpain |
Autolysis |
34 kDa, 21 kDa |
Large subunit of Bos, u-calpain |
5821 |
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Autolysis |
34 kDa, 22 kDa |
Large subunit of Bos, u-calpain |
5821 |
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Autolysis |
18 kDa |
Small subunit of Bos |
5821 |
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|
|
Autolysis |
18-528, 18-407, 408, 705, 529-705/88-266 |
Large/small subunit of Gallus |
1825 |
|
21 |
Sorcine |
|
|
|
|
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22 |
S100, intestinal calcium-binding protein |
Trypsin |
|
Sequence determination (ICBP) |
|
|
|
|
Chymotrypsin |
|
Sequence determination (ICBP) |
|
|
|
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Pepsin |
|
Sequence determination (ICBP) |
|
|
|
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0.25M Acetic acid |
|
Sequence determination (ICBP) |
|
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|
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Trypsin |
|
Calcium-binding (ICBP) |
2157 |
|
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Trypsin |
|
Sequence determination (S100) |
|
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BrCN |
|
Sequence determination (S100) |
|
|
28 |
Alfa-Actinin |
Thermolysin |
27 kDa/53 kDa |
Actin-binding site/Beta1 integrin binding |
3795, 3795 |
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|
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Calpain |
|
|
2941 |
|
|
|
Trypsin |
|
F-actin cross-linking |
2499 |
|
43 |
Calcium-dependent protein kinase |
Trypsin |
55 kDa |
Membrane association |
|
|
45 |
Strongylocentrotus purpuratus ectodermal |
Lysylendopeptidase |
|
Sequence determination |
|
|
|
calcium-binding protein |
|
|
|
|
|
|
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S. aureus V8 protease |
|
Sequence determination |
|
|
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|
BrCN |
|
Sequence determination |
|
|
48 |
Sarcoplasmic calcium-binding protein |
Lysylendopeptidase |
|
Sequence determination |
|
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|
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Trypsin |
|
Sequence determination |
|
|
|
|
BrCN |
|
Sequence determination |
|
|
|
|
Trypsin |
1-18, 90-174 |
Calcium-binding |
294 |
|
53 |
Calcium vector protein |
Endoproteinase-Arg C |
|
Sequence determination |
|
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Trypsin |
|
Sequence determination |
|
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|
Lysylendopeptidase |
|
Sequence determination |
|
|
|
|
Pepsin |
|
Sequence determination |
|
|
|
|
S. aureus V8 protease |
|
Sequence determination |
|
|
58 |
Parvalbumin |
Trypsin |
|
Sequence determination |
|
|
|
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Chymotrypsin |
|
Sequence determination |
|
|
|
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N-bromosuccinimide |
|
Sequence determination |
|
|
|
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BrCN |
|
Sequence determination |
|
|
|
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Carboxypeptidase |
C-terminus |
NMR |
1802 |
|
|
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BrCN |
1-37, 38-108 |
Calcium-binding |
377, 3822 |
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Trypsin |
|
Sequence determination (oncomodulin) |
|
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BrCN |
|
Sequence determination (oncomodulin) |
|
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Trypsin |
|
Sequence determination (avian thymic hormone) |
|
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|
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S. aureus V8 protease |
|
Sequence determination (avian thymic hormone) |
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