Our laboratory works at the nexus of environmental microbiology, analytical chemistry and toxicology with two overarching themes:<\/p>\n\n\n\n
1) Investigation of microorganisms that transform hazardous environmental pollutants with special attention to petroleum hydrocarbons such as polycyclic aromatic hydrocarbons (PAHs) and heterocyclic PAHs.<\/strong><\/p>\n\n\n\n – Mechanisms of organic pollutant transformation 2) Investigation of the effects of hazardous chemical exposure to nucleic acids through the formation of DNA and RNA modifications.<\/strong><\/p>\n\n\n\n -Development of the fields of DNA and RNA adductomics <\/a><\/p>\n\n\n\n Group Leader<\/strong><\/p>\n\n\n\n Robert A. Kanaly, Ph.D.\u3000\u3000\u30ab\u30ca\u30ea\u30fc\u3000\u30ed\u30d0\u30fc\u30c8<\/strong><\/p>\n\n\n\n Professor, Yokohama City University, Japan<\/p>\n\n\n\n Peer-reviewed articles \/ \u67fb\u8aad\u4ed8\u304d\u5b66\u8853\u8ad6\u6587<\/strong><\/p>\n\n\n\n 2025<\/strong><\/p>\n\n\n\n Sakai, M., Mori, J. F., & Kanaly, R. A.<\/strong> (2025). Utilization of the C9 aromatic hydrocarbon n<\/em>-propylbenzene by Sphingobium barthaii<\/em> KK22 and coexistence of multiple biodegradation pathways.\u00a0Biodegradation<\/a><\/strong><\/em>,\u00a036<\/em>.<\/p>\n\n\n\n Yanagita, H., Kanaly, R. A.<\/strong>, & Mori, J. F. (2025). Transcriptomic profiling of Pseudomonas migulae<\/em> revealed gene regulatory properties during biodegradation of aromatic hydrocarbons under cold stress.\u00a0Microbial Genomics<\/strong><\/em>,\u00a011<\/em><\/a>.<\/p>\n\n\n\n Abe, M., Sakai, M., Kanaly, R. A.<\/strong>, & Mori, J. F. (2025). Identification of a putative novel polycyclic aromatic hydrocarbon-biodegrading gene cluster in a marine Roseobacteraceae<\/em> bacterium Sagittul<\/em>a sp. MA-2.\u00a0Microbiology Spectrum<\/strong><\/em>,\u00a013<\/em><\/a>.<\/p>\n\n\n\n Yamakami, Y., Morino, K., Kanaly, R. A.<\/strong>, Irie, R., Oikawa, M., Takauji, Y., Miki, K., Hossain, M., Ayusawa, D.,\u00a0& Fujii, M. (2025). 1,6-Digalloyl glucose in Emblica officinalis<\/em> increases the proliferation of human keratinocytes and fibroblasts with upregulated expression of periostin.\u00a0Bioscience, Biotechnology, and Biochemistry<\/strong><\/em>, 89<\/em><\/a>.<\/p>\n\n\n\n 2024<\/strong><\/p>\n\n\n\n Sakai, M., Mori, J. F., & Kanaly, R. A.<\/strong> (2024). Assessment of bacterial biotransformation of alkylnaphthalene lubricating base oil component 1-butylnaphthalene by LC\/ESI-MS (\/MS).\u00a0Chemosphere<\/strong><\/em>,\u00a0364<\/em><\/a>.<\/p>\n\n\n\n Tsushima, S., Nishi, Y., Suzuki, R., Tachibana, M., Kanaly, R. A.<\/strong>, & Mori, J. F. (2024). Formation of biogenic manganese oxide nodules on hyphae of a new fungal isolate of Periconia<\/em> that immobilizes aqueous copper.\u00a0Microbes and Environments<\/strong><\/em>,\u00a039<\/em><\/a>.<\/p>\n\n\n\n 2023<\/strong><\/p>\n\n\n\n Nemoto, Y., Ozawa, K., <\/strong>Mori, J. F., and Kanaly, R. A.<\/strong> (2023) Nondesulfurizing benzothiophene biotransformation to hetero and homodimeric ortho<\/em>-substituted diaryl disulfides by the model PAH-degrading Sphingobium barthaii<\/em>. Biodegradation<\/a><\/strong><\/em>, 34.<\/p>\n\n\n\n Toida, K., Kushida, W., Yamamoto, H., Yamamoto, K., Ishii, K., Uesaka, K., Kanaly, R. A.<\/strong>, Kutsuna, S., Ihara, K., Fujita, Y. (2023) The GGDEF protein Dgc2 suppresses both motility and biofilm formation in the filamentous cyanobacterium Leptolyngbya boryana<\/em>. Microbiology Spectrum<\/strong><\/em>, 11<\/a>.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n\n\n\n Tsushima, S., Kanaly, R. A.<\/strong>, and Mori, J. F. (2023) Whole-genome sequence of Periconia<\/em> sp. strain TS-2, an ascomycete fungus isolated from a freshwater outflow and capable of Mn(II) oxidation. Microbiology Resource Announcements<\/strong><\/em>, 12<\/a>.\u00a0\u00a0\u00a0\u00a0<\/p>\n\n\n\n Abe, M., Kanaly, R. A.<\/strong>, and Mori, J. F. (2023) Genomic analysis of a marine alphaproteobacterium Sagittula<\/em> sp. strain MA-2 that carried eight plasmids. Marine Genomics<\/strong><\/em>, 72<\/a>.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n\n\n\n 2022<\/strong><\/p>\n\n\n\n Kayama, G., Kanaly, R. A.<\/strong>, and Mori, J. F. (2022) Comprehensive genomic characterization of marine bacteria Thalassospira<\/em> spp. provides insights into their ecological roles in aromatic hydrocarbon-exposed environments. Microbiology Spectrum<\/strong><\/em>, 10.<\/a><\/p>\n\n\n\n Sakai, M., Tomiyama, Y., Mori, J. F., and Kanaly, R. A.<\/strong> (2022) Growth of Sphingobium barthaii<\/em> KK22 on 1-ethylnaphthalene reveals diverse oxidative transformations and a complex metabolite profile. International Biodeterioration & Biodegradation<\/strong><\/em>, 175<\/a>.<\/p>\n\n\n\n Kameda, M., Kanaly, R. A.<\/strong>, Harada, M., Aoki, S., Tsukada, H., and Kutsuna, S. (2022) Quantification of cyanobacterial cyclic di-guanosine monophosphate (c-di-GMP) by liquid chromatography electrospray ionization tandem mass spectrometry. Journal of Microbiological Methods<\/strong><\/em>, 196<\/a>.<\/p>\n\n\n\n Kayama, G., Kanaly, R. A.<\/strong>, and Mori, J. F.<\/strong><\/strong> (2022) Complete genome sequence of Thalassospira<\/em> sp. strain GO-4, a marine bacterium isolated from a phenanthrene-enriched bacterial consortium. <\/strong>Microbiology Resource Announcements<\/strong><\/em><\/strong><\/em><\/strong>, 11<\/a>.<\/p>\n\n\n\n Mori, J. F. and Kanaly, R. A.<\/strong> (2022) Natural chromosome-chromid fusion across rRNA operons in a Burkholderiaceae<\/em> bacterium. Microbiology Spectrum<\/strong><\/em>, 10<\/a>.<\/p>\n\n\n\n 2021<\/strong><\/p>\n\n\n\n Izawa, M., Sakai, M., Mori, J. F., and Kanaly, R. A.<\/strong> (2021) Cometabolic benzo[a<\/em>]pyrene biotransformation by Sphingobium barthaii<\/em> KK22 proceeds through the kata-annelated ring and 1-pyrenecarboxylic acid to downstream products. Journal of Hazardous Materials Advances<\/strong><\/em>, 4<\/a>.<\/p>\n\n\n\n Mori, J. F., Nagai, M., and Kanaly, R. A.<\/strong> (2021) Complete genome sequence of Cupriavidus necator<\/em> KK10, an azaarene-degrading and polyhydroxyalkanoate-producing soil bacterium. Microbiology Resource Annoucements<\/strong><\/em>, 10<\/a>.<\/p>\n\n\n\n Tomiyama, Y., Takeshita, T., Mori, J. <\/strong>F. and Kanaly, R. A.<\/strong> (2021) Functionalization of the model asphaltene 1-dodecylnaphthalene by Pseudomonas aeruginosa <\/em>KK6 through subterminal metabolism. Journal of Petroleum Science and Engineering<\/strong><\/em>, <\/a><\/strong><\/em>205<\/a>.<\/p>\n\n\n\n Matsumoto, A., Koga, R., Kanaly, R. A.<\/strong>, Kouzuma, A., and Watanabe, K. (2021) Identification of a diguanylate cyclase that facilitates biofilm formation on electrodes by Shewanella oneidensis<\/em> MR-1. Applied and Environmental Microbiology<\/strong><\/em>, 87<\/a>.<\/p>\n\n\n\n Mori, J. F. and Kanaly, R. A.<\/strong> (2021) Complete genome sequence of Sphingobium barthaii<\/em> KK22, a high-molecular-weight polycyclic aromatic hydrocarbon-degrading soil bacterium. Microbiology Resource Announcements<\/em><\/strong>, 10<\/a>.<\/p>\n\n\n\n 2020<\/strong><\/p>\n\n\n\n Mori, J. F. and Kanaly, R. A.<\/strong> (2020) Multispecies diesel fuel biodegradation and niche formation are ignited by pioneer hydrocarbon-utilizing proteobacteria in a soil bacterial consortium. Applied and Environmental Microbiolo<\/strong><\/a><\/em>gy<\/strong><\/em>, 87<\/a>.<\/p>\n\n\n\n Maeda, A. H., Nishi, S., Hatada, Y., Ohta, Y., Misaka, K., Kunihiro, M., Mori, J. F., and Kanaly, R. A.<\/strong> (2020) Chemical and genomic analyses of polycyclic aromatic hydrocarbon biodegradation in Sphingobium barthaii<\/em> KK22 reveals divergent pathways in soil sphingomonads. International Biodeterioration & Biodegradation<\/em><\/strong>, 151<\/a>.<\/p>\n\n\n\n Matsui, T., Yamada, N., Kuno, H., and Kanaly, R. A.<\/strong> (2020) Characterization of N<\/em>-(2,6-dimethylphenyl)hydroxylamine adducts of 2′-deoxyguanosine under weakly basic conditions. Chemosphere, <\/em><\/strong>252<\/a>.<\/p>\n\n\n\n 2019<\/strong><\/p>\n\n\n\n Matsui, T., Yamada, N., Kuno, H., and R. A. Kanaly<\/strong> (2019) Formation of bulky DNA adducts by non-enzymatic production of 1,2-naphthoquinone-epoxide from 1,2-naphthoquinone under physiological conditions. Chemical Research in Toxicology<\/em><\/strong>, 32<\/a>.<\/em><\/p>\n\n\n\n Takeshita, T. and R. A. Kanaly<\/strong> (2019) In vitro<\/em> DNA\/RNA adductomics to confirm DNA damage caused by benzo[a<\/em>]pyrene in the Hep G2 cell line. Frontiers in Chemistry, Analytical Chemistry<\/em><\/strong>, 7<\/a>.<\/p>\n\n\n\n Takeshita, T., Tao, F., Kojima, N., and R. A. Kanaly<\/strong> (2019) Triple quadrupole mass spectrometry comparative DNA adductomics of Hep G2 cells following exposure to safrole. Toxicology Letters<\/a><\/strong><\/em>,<\/a> 300.<\/a><\/p>\n\n\n\n 2008-2018<\/strong><\/p>\n\n\n\n Kobayashi, T., Obana, Y., Kuboi, N., Kitayama, Y., Hayashi, S., Oka, M., Wada, N., Arita, K., Shimizu, T., Sato, M., Kanaly, R. A.<\/strong>, and S. Kutsuna. (2016) Analysis of the fine-tuning of cyanobacterial circadian phase by monochromatic light and long-day conditions. Plant and Cell Physiology, <\/a><\/strong><\/em>57.<\/a><\/p>\n\n\n\n Fukuoka, K., Tanaka, K., Ozeki, Y. and and R. A. Kanaly<\/strong> (2015) Biotransformation of indole by Cupriavidus<\/em> sp. strain KK10 proceeds through N<\/em>-heterocyclic and carbocyclic-aromatic ring cleavage and production of indigoids. International Biodeterioration & Biodegradation<\/a><\/strong><\/em>,<\/a> 97<\/a>.<\/p>\n\n\n\n Kanaly, R. A.<\/strong>, Micheletto, R., Matsuda, T., Utsuno, Y., Ozeki, Y. and N. Hamamura (2015) Application of DNA adductomics to a soil bacterium Sphingobium<\/em> sp. strain KK22. MicrobiologyOpen<\/strong><\/a><\/em>,<\/a> 4.<\/a><\/p>\n\n\n\n Fukuoka, K., Ozeki, Y., and R. A. Kanaly<\/strong> (2015) Aerobic biotransformation of 3-methylindole to ring cleavage products by Cupriavidus<\/em> sp. strain KK10. Biodegradation<\/strong><\/em>, 26<\/a>.<\/p>\n\n\n\n Maeda, A.H., Kunihiro, M., Ozeki, Y., Nogi, Y. and R. A. Kanaly<\/strong> (2015) Sphingobium barthaii<\/em> sp. nov., a high molecular weight polycyclic aromatic hydrocarbon-degrading bacterium isolated from cattle pasture soil. International Journal of Systematic and Evolutionary Microbiology<\/a><\/strong><\/em> 65:2919-2924<\/a>.<\/p>\n\n\n\n Maeda, A.H., Nishi, S., Hatada, Y., Ozeki, Y. and R. A. Kanal<\/strong>y (2014) Biotransformation of the HMW PAH benzo[k<\/em>]fluoranthene by Sphingobium<\/em> sp. strain KK22 and identification of new products of non-alternant PAH biodegradation by LC\/ESI-MS\/MS analyses. Microbial Biotechnology<\/a><\/strong><\/em> 7:114-129<\/a>.<\/p>\n\n\n\n Kunihiro, M., Ozeki, Y., Nogi, Y., Hamamura, N. and R. A. Kanaly<\/strong> (2013) Benz[a<\/em>]anthracene biotransformation and production of ring fission products by Sphingobium<\/em> sp. strain KK22. Applied and Environmental Microbiology<\/a><\/strong><\/em> 79:4410-4420<\/a>.<\/p>\n\n\n\n Maeda, A.H., Nishi, S., Ozeki, Y., Ohta, Y., Hatada, Y. and R. A. Kanaly<\/strong> (2013) Draft genome sequence of Sphingobium<\/em> sp. strain KK22, a high-molecular-weight polycyclic aromatic hydrocarbon-degrading bacterium isolated from cattle pasture soil.
– Bioremediation of oil hydrocarbons
– Functionalization of heavy oil hydrocarbons in the context of oil upgrading
– Biodiesel waste glycerol conversion to polyhydroxyalkanoates (PHA) by bacteria<\/p>\n\n\n\n
-Chemical characterization of DNA and RNA adducts<\/p>\n\n\n\n